From sediment to data: Collaborative sample analysis by UKSH Toxicology
The journey of sediment samples from collection to data analysis is a detailed and precise process conducted by UKSH Toxicology. These samples, originating from the Flanders Marine Institute (VLIZ), are carefully processed to ensure high-quality results and meaningful insights.
The process begins with sediment samples stored in 50 ml tubes in a freezer. These samples are freeze-dried, bundled into "cakes," and aliquoted into labeled 15 ml tubes with two technical replicates per sample. Solid phase extraction columns, carefully labeled for each aliquot and control, play a central role in the analysis.
The samples are treated with organic solvents and placed in an ultrasonic bath to ensure thorough extraction. Centrifugation follows, separating sediment from solvent, and the supernatant is processed through Solid phase extraction columns using a vacuum chamber. The columns, frozen after the initial processing, are later thawed, dried, and eluted with acetonitrile. The eluate is concentrated and adjusted to precisely 1 ml before being transferred to amber glass vials.
Finally, the samples are loaded into the cooling drawer of the GC-MS (Gas Chromatography-Mass Spectrometry), where detailed measurements of the samples and standards are performed. This generates critical data for further analysis, providing insights into sediment composition and its environmental implications.
This meticulous workflow ensures that the samples are processed with care and precision, reflecting the strong collaboration between REMARCO’s partner institutions in studying and protecting marine environments.
From samples pickup by colleague Jacqueline Lindemeyer to processing with Easy columns in the vacuum chamber © Maren Gundlach, UKSH 2024. All rights reserved.
From frozen columns in storage to GC-MS measurements and detailed data analysis © Maren Gundlach, UKSH 2024. All rights reserved.
Cover: © Klara Liebrecht, AWI 2024. All rights reserved.